Department: Research Support Office

Campus: Camden

Research Groups: Host-Pathogen Interactions and Vaccinology

Research Centres: RVC Quantitative Biology Resource

Dong is a lecturer in bioinformatics who contributes to various research activities in the College while leading his own research. Dong's research interests focus on understanding the molecular mechanisms of host-pathogen interactions using high-resolution approaches coupled to bioinformatics. He is interested in 'big data' analysis, where datasets generated from multiple omics platforms are integrated for system modelling.

Dong obtained his Biological Science degree from Shandong University in China followed by MSc by research training in the University of Edinburgh. He has then enrolled a PhD program in the School of Veterinary Science, University of Liverpool on infection biology to characterise the proteomics of Toxoplasma gondii.

After completing his PhD, Dong has led and involved in various bioinformatics and wet lab based projects such as developing informatics tools for mass spectrometry data analysis, genome annotation improvements and characterising molecular mechanisms of host-pathogen interactions in several important veterinary and human infectious diseases, ranging from protozoan parasites, bacteria, worms and viruses.

In 2017, he was appointed to a lectureship at the Royal Veterinary College in the University of London, where he continues his research interests in understanding the molecular mechanisms of host-pathogen interactions using multi-omics approaches coupled to bioinformatics and contributes to the RVC Quantitative Biology Resource.

Dong's research interests focus on understanding the molecular mechanisms of host-pathogen interactions using high-resolution approaches coupled to bioinformatics. He is interested in 'big data' analysis, where datasets generated from multiple omics platforms are integrated for system modelling.

Trained as an experimental biologist with hands-on mass spectrometer and bioinformatics skills, his work also contributes to the understanding of proteoforms, where splice variants and post translational modifications are experimentally and bioinformatically characterised followed by functional validations. The data generated also contribute to the next round of genome annotation as solid evidence of expression to improve gene model predictions.

Google Scholar Page (https://scholar.google.co.uk/citations?user=QImkpV0AAAAJ&hl=en)

 

Dubourg A, Xia D, Winpenny JP, Naimi SA, Bouzid M, Sexton DW, Wastling JM, Hunter PR, Tyler KM. (2017). "Giardia Secretome Highlights Secreted Tenascins as a Key Component of Pathogenesis" Gigascience. 2018 Jan 29. doi: 10.1093/gigascience/giy003

Dong, X., Armstrong, S. D., Xia, D., Makepeace, B. L., Darby, A. C., Kadowaki, T. (2017). "Draft genome of the honey bee ectoparasitic mite, Tropilaelaps mercedesae, is shaped by the parasitic life history." GigaScience doi: 10.1093/gigascience/gix008

Liu, X., E. Speranza, C. Muñoz-Fontela, S. Haldenby, N. Y. Rickett, I. Garcia-Dorival, Y. Fang, Y. Hall, E.-G. Zekeng and A. Lüdtke (2017). "Transcriptomic signatures differentiate survival from fatal outcomes in humans infected with Ebola virus." Genome Biology 18(1): 4.

Armstrong, S. D., D. Xia, G. S. Bah, R. Krishna, H. F. Ngangyung, E. J. LaCourse, H. J. McSorley, J. A. Kengne-Ouafo, P. W. Chounna-Ndongmo and S. Wanji (2016). "Stage-specific proteomes from Onchocerca ochengi, sister species of the human river blindness parasite, uncover adaptations to a nodular lifestyle." Molecular & Cellular Proteomics 15(8): 2554-2575.

Borgdorff, H., S. D. Armstrong, H. L. Tytgat, D. Xia, G. F. Ndayisaba, J. M. Wastling and J. H. van de Wijgert (2016). "Unique Insights in the Cervicovaginal Lactobacillus iners and L. crispatus Proteomes and Their Associations with Microbiota Dysbiosis." PloS one 11(3): e0150767.

Dong, S., L. Liu, W. Wu, S. D. Armstrong, D. Xia, H. Nan, J. A. Hiscox and H. Chen (2016). "Determination of the interactome of non-structural protein12 from highly pathogenic porcine reproductive and respiratory syndrome virus with host cellular proteins using high throughput proteomics and identification of HSP70 as a cellular factor for virus replication." Journal of Proteomics 146: 58-69.

Hunt, V. L., I. J. Tsai, A. Coghlan, A. J. Reid, N. Holroyd, B. J. Foth, A. Tracey, J. A. Cotton, E. J. Stanley and H. Beasley (2016). "The genomic basis of parasitism in the Strongyloides clade of nematodes." Nature genetics. 2016 Mar;48(3):299-307.

Wang, K., E. D. Peng, A. S. Huang, D. Xia, S. J. Vermont, G. Lentini, M. Lebrun, J. M. Wastling and P. J. Bradley (2016). "Identification of Novel O-Linked Glycosylated Toxoplasma Proteins by Vicia villosa Lectin Chromatography." PloS one 11(3): e0150561.

Xiao, Y., W. Wu, J. Gao, N. Smith, C. Burkard, D. Xia, M. Zhang, C. Wang, A. Archibald and P. Digard (2016). "Characterization of the Interactome of the Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 2 Reveals the Hyper Variable Region as a Binding Platform for Association with 14–3–3 Proteins." Journal of proteome research 15(5): 1388-1401.

Borgdorff, H., R. Gautam, S. Armstrong, D. Xia, G. Ndayisaba, N. van Teijlingen, T. Geijtenbeek, J. Wastling and J. van de Wijgert (2015). "Cervicovaginal microbiome dysbiosis is associated with proteome changes related to alterations of the cervicovaginal mucosal barrier." Mucosal immunology. 2016 May;9(3):621-33.

Dent, S. D., D. Xia, J. M. Wastling, B. W. Neuman, P. Britton and H. J. Maier (2015). "The proteome of the infectious bronchitis virus Beau-R virion." Journal of General Virology 96(12): 3499-3506.

Jackson, A. P., S. Goyard, D. Xia, B. J. Foth, M. Sanders, J. M. Wastling, P. Minoprio and M. Berriman (2015). "Global Gene Expression Profiling through the Complete Life Cycle of Trypanosoma vivax." PLoS Negl Trop Dis 9(8): e0003975.

Krishna, R., D. Xia, S. Sanderson, A. Shanmugasundram, S. Vermont, A. Bernal, G. Daniel-Naguib, F. Ghali, B. P. Brunk and D. S. Roos (2015). "A large-scale proteogenomics study of apicomplexan pathogens—Toxoplasma gondii and Neospora caninum." Proteomics 15(15): 2618-2628.

Armstrong, S. D., S. A. Babayan, N. Lhermitte-Vallarino, N. Gray, D. Xia, C. Martin, S. Kumar, D. W. Taylor, M. L. Blaxter and J. M. Wastling (2014). "Comparative analysis of the secretome from a model filarial nematode (Litomosoides sigmodontis) reveals maximal diversity in gravid female parasites." Molecular & Cellular Proteomics 13(10): 2527-2544.

Darby, A. C., A. C. Gill, S. D. Armstrong, C. S. Hartley, D. Xia, J. M. Wastling and B. L. Makepeace (2014). "Integrated transcriptomic and proteomic analysis of the global response of Wolbachia to doxycycline-induced stress." The ISME journal 8(4): 925-937.

Ghali, F., R. Krishna, S. Perkins, A. Collins, D. Xia, J. Wastling and A. R. Jones (2014). "ProteoAnnotator–Open source proteogenomics annotation software supporting PSI standards." Proteomics 14(23-24): 2731-2741.

Jackson, A. P., T. D. Otto, A. Darby, A. Ramaprasad, D. Xia, I. E. Echaide, M. Farber, S. Gahlot, J. Gamble and D. Gupta (2014). "The evolutionary dynamics of variant antigen genes in Babesia reveal a history of genomic innovation underlying host–parasite interaction." Nucleic acids research 42(11): 7113-7131.

Wiens, O., D. Xia, C. von Schubert, J. M. Wastling, D. A. Dobbelaere, V. T. Heussler and K. L. Woods (2014). "Cell cycle-dependent phosphorylation of Theileria annulata schizont surface proteins." PloS one 9(7): e103821.

Doliwa, C., D. Xia, S. Escotte-Binet, E. L. Newsham, D. Aubert, N. Randle, J. M. Wastling and I. Villena (2013). "Identification of differentially expressed proteins in sulfadiazine resistant and sensitive strains of Toxoplasma gondii using difference-gel electrophoresis (DIGE)." International Journal for Parasitology: Drugs and Drug Resistance 3: 35-44.

Wastling, J. and D. Xia (2013). Proteomics of Toxoplasma gondii, Toxoplasma gondii: The Model Apicomplexan-Perspectives and Methods: Second Edition. Amsterdam and Boston: Elsevier/Academic Press.

Witschi, M., D. Xia, S. Sanderson, M. Baumgartner, J. Wastling and D. Dobbelaere (2013). "Proteomic analysis of the Theileria annulata schizont." International journal for parasitology 43(2): 173-180.

Qi, D., P. Brownridge, D. Xia, K. Mackay, F. F. Gonzalez-Galarza, J. Kenyani, V. Harman, R. J. Beynon and A. R. Jones (2012). "A software toolkit and interface for performing stable isotope labeling and top3 quantification using Progenesis LC-MS." Omics: a journal of integrative biology 16(9): 489-495.

Wastling, J., S. Armstrong, R. Krishna and D. Xia (2012). "Parasites, proteomes and systems: has Descartes’ clock run out of time?" Parasitology 139(09): 1103-1118.

Xia, D., F. Ghali, S. J. Gaskell, R. O'Cualain, P. F. Sims and A. R. Jones (2012). "Software for analysing ion mobility mass spectrometry data to improve peptide identification." Proteomics 12(12): 1912-1916.

Sohn, C. S., T. T. Cheng, M. L. Drummond, E. D. Peng, S. J. Vermont, D. Xia, S. J. Cheng, J. M. Wastling and P. J. Bradley (2011). "Identification of novel proteins in Neospora caninum using an organelle purification and monoclonal antibody approach." PLoS One 6(4): e18383.

Wastling, J., D. Xia, A. Sohal, M. Chaussepied, A. Pain and G. Langsley (2009). "Proteomes and transcriptomes of the Apicomplexa–Where’s the message?" International journal for parasitology 39(2): 135-143.

Wu, Y., M. M. Nelson, A. Quaile, D. Xia, J. M. Wastling and A. Craig (2009). "Identification of phosphorylated proteins in erythrocytes infected by the human malaria parasite Plasmodium falciparum." Malaria journal 8(1): 105.

Sanderson, S. J., D. Xia, H. Prieto, J. Yates, M. Heiges, J. C. Kissinger, E. Bromley, K. Lal, R. E. Sinden and F. Tomley (2008). "Determining the protein repertoire of Cryptosporidium parvum sporozoites." Proteomics 8(7): 1398-1414.

Xia, D., S. J. Sanderson, A. R. Jones, J. H. Prieto, J. R. Yates, E. Bromley, F. M. Tomley, K. Lal, R. E. Sinden and B. P. Brunk (2008). "The proteome of Toxoplasma gondii: integration with the genome provides novel insights into gene expression and annotation." Genome biology 9(7): R116.

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