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c) If insufficient serum is received, we will either request more serum, or
perform those tests deemed appropriate by the pathologist, if order of
preference is not stated on the request form.
d) Serum is preferred, especially for bile acid analysis. Plasma (heparin) is
suitable for reptiles and most birds.

CYTOLOGY (AVOID CONTACT WITH FORMALIN)
a) Fluids
Submit in EDTA (prevents clotting if blood present)
• Make direct smears if sample is not to be received on collection day.
• Send unstained smears.
• CSF - contact laboratory for sampling requirements.

b) Impression smears
• Make gentle imprints, blotting excess blood and scoring surface
with a scalpel blade if exfoliation is difficult.
• Send unstained smears.

c) Aspirate smears
• Send unstained smears.
• Do not refrigerate smears. Tubes can be refrigerated if there is a
delay in testing.

If possible, send 3-5 unstained slides (additional stains may be
required for a diagnosis).

PT/APTT (PLEASE CONTACT LAB BEFORE SUBMITTING SAMPLES)
• Tubes (Sodium Citrate).
• Required volume stated on the tubes is critical.
• A non-haemolysed, non-lipemic sample is
required - needle removal prior to expulsion of
blood into tube is recommended. Mix gently.
• A “normal” species control may be required due
to changing reagents - please confirm with lab.
• Bring sample to lab as soon as possible. If there
has to be a delay, separate plasma and place
sample on ice pack.

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