Department: Clinical Sciences and Services

Campus: Hawkshead

Research Groups: Musculoskeletal Biology

Jay is a Senior Lecturer in Matrix Biology in the Department of Veterinary Clinical Sciences. His research interest in the ageing of soft tissue and involves investigations into equine tendon disease and in human osteoarthitis with a focus on the age-associated alterations of extracellular matrices and markers of early disease.

Jay graduated from Queen Elizabeth College, University of London with a BSc Honours degree in Biochemistry and Physiology and then gained a PhD in 1984 in Molecular Biology at Queen Elizabeth College (Kings College London). He took up a postdoctoral position Professor Tim Hardingham in the Department of Biochemistry at the Kennedy Institute of Rheumatology in London to pursue interests in the molecular changes in articular cartilage with ageing and in osteoarthritis, and the cloning and sequencing of a number of extracellular matrix proteins.

In 1997 he joined The Royal Veterinary College as a Senior Research Fellow to continue studies on articular and intervertebral disc cartilages in Professor Mike Bayliss' group in the Department of Veterinary Basic Sciences. It was at this time that his research interest into tendon disease in the horse developed in collaborative work with Professor Roger Smith to investigate markers of early tendon disease. In 2003 he moved to the Department of Veterinary Clinical Sciences to continue work on age- and exercise-induced mechanisms of tendon disease and the opportunity to work closely with the Equine Clinic has led to developing an interest in stem cell research and the application of this technology in Clinic. Jay's research on osteoarthritis has continued and he is currently developing Raman spectroscopy as a minimally invasive method of assessing early cartilage disease.

He holds an Honorary Senior Lectureship at University College London. He is a member of a number of national societies including the British Society for Matrix Biology where he has previously served as a Committee and Board Member, the Tissue and Cell Engineering Society and the British Society for Cell Biology. He is an Editorial Board Member of the Open Geriatric Medicine Journal. He also acts as a Consultant to Industry and academic Institutions in the area of genetic modified organisms (GMOs) and related health and safety aspects.
 

The main area of Jay's research is on the extracellular matrices of articular cartilage and tendons and the mechanisms of ageing and exercise that lead to osteoarthritis and tendinopathy.

His research in osteoarthritis has concentrated on the disease in man and has recently been investigating the potential of Raman spectroscopy to assess molecular alterations of the cartilage in very early disease prior to signs of erosion. This work has led to Jay leading a pilot clinical trial in which the successfully technology was applied via minimally invasive surgery in the knees of osteoarthritis patients. Together with collaborators at University College London and Charing Cross Hospital, Imperial College, this technology is now being taken forward for further technological and commercial development.

His research on tendon has two aims: identifying disease mechanisms and developing clinical application of stem cell based therapies. A major part of this research is in tendinitis occurring in athletic horses and he also is developing serological markers of the disease with equine clinical colleagues.
 

 

Yamamoto K, Owen K, Parker AE, Scilabra SD, Dudhia J, Strickland DK, Troeberg L, Nagase H. (2014) Low Density Lipoprotein Receptor-related Protein 1 (LRP1)-mediated Endocytic Clearance of a Disintegrin and Metalloproteinase with Thrombospondin Motifs-4 (ADAMTS-4): FUNCTIONAL DIFFERENCES OF NON-CATALYTIC DOMAINS OF ADAMTS-4 AND ADAMTS-5 IN LRP1 BINDING. J Biol Chem. 2014 Mar 7;289(10):6462-74.

Dakin SG, Dudhia J and Smith RK (2014) Resolving an inflammatory concept: the importance of inflammation and resolution in tendinopathy. Vet Immunol Immunopathol. 2014 Apr 15;158(3-4):121-7.

Dakin SG, Smith RK, Heinegård D, Önnerfjord P, Khabut A and Dudhia J. (2014) Proteomic analysis of tendon extracellular matrix reveals disease stage-specific fragmentation and differential cleavage of COMP (cartilage oligomeric matrix protein). J Biol Chem. 2014 Feb 21;289(8):4919-27.

Smith RK, Werling NJ, Dakin SG, Alam R, Goodship AE, Dudhia J. (2013) Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy. PLoS One. Sep 25;8(9):e75697. doi: 10.1371/journal.pone.0075697.

Dakin SG, Dudhia J, Smith RK. (2013). Science in brief: Resolving tendon inflammation. A new perspective. Equine Vet J. Jul;45(4):398-400.

Becerra P, Valdés Vázquez MA, Dudhia J, Fiske-Jackson AR, Neves F, Hartman NG, Smith RKW. (2013). Distribution of Injected Technetium99m-Labelled Mesenchymal Stem Cells in Horses with Naturally Occurring Tendinopathy. Jul;31(7):1096-102.

Manning HB, Nickdel MB, Yamamoto K, Lagarto JL, Kelly DJ, Talbot CB, Kennedy G, Dudhia J, Lever J, Dunsby C, French P, Itoh Y. (2013) Detection of cartilage matrix degradation by autofluorescence lifetime. Matrix Biol. Jan;32(1):32-8.

Dakin, SG; Dudhia, J; Werling, NJ; Werling, D; Abayasekara, DRE; Smith, RKW. (2012)  Inflamm-Aging and Arachadonic Acid Metabolite Differences with Stage of Tendon Disease.  PLoS One, 7 (11):e48978.

Dakin SG, Werling D, Hibbert A, Abayasekara DRE, Young NJ, Smith RKW, Dudhia J (2012) Macrophage sub-populations and the Lipoxin A4 receptor implicate active inflammation during equine tendon repair. PLoS One. 7(2):e32333.

Godwin EE, Young NJ, Dudhia J, Beamish IC, Smith RK. (2012) Implantation of bone marrow-derived mesenchymal stem cells demonstrates improved outcome in horses with overstrain injury of the superficial digital flexor tendon. Equine Vet J. 2012 Jan;44(1):25-32.

Alves AG, Stewart AA, Dudhia J, Kasashima Y, Goodship AE, Smith RK. (2011) Cell-based therapies for tendon and ligament injuries. Vet Clin North Am Equine Pract. Aug;27(2):315-33.

Dakin SG, Jespers S, Warner S, O’Hara LK, DudhiaJ, Goodship AE, Wilson AM, Smith RKW ‘The relationship between in vivo limb and in vitro tendon mechanics after injury: A potential novel clinical tool for monitoring tendon repair’ (2011) Equine vet. J. 43(4):418-423

Smith MR, Wright IM, Minshall GJ, Dudhia J, Verheyen K, Heinegård D, Smith RK. (2011) Increased Cartilage Oligomeric Matrix Protein Concentrations in Equine Digital Flexor Tendon Sheath Synovial Fluid Predicts Intrathecal Tendon Damage. Vet Surg. 2011 Jan;40(1):54-58

Dart AJ, Dart CM, Dudhia J, Perkins N, Canfield P, Smith RK. (2011) A Preliminary Study on the Effect of Wounding on Transforming Growth Factor-β1 and Cartilage Oligomeric Matrix Protein Concentrations in the Skin of Horses. Vet Surg. Jan;40(1):59-65

Williams R, Khan IM, Richardson K, Nelson L, McCarthy HE, Analbelsi T, Singhrao SK, Dowthwaite GP, Jones RE, Baird DM, Lewis H, Roberts S, Shaw HM, Dudhia J, Fairclough J, Briggs T, Archer CW (2010) Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage. PLoS One. 14;5(10):e13246.

Briston L, Dudhia J and Lees P (2009) Age-related differences in prostaglandin E2 synthesis by equine cartilage explants and synoviocytes. J Vet Pharmacol Ther. 2010 Jun 1;33(3):268-76.

Miller M-C, Manning HB, Jain A, Troeberg L, Dudhia J, Essex D, Sandison A, Seiki M, Nanchahal J, Nagase H and Itoh Y (2009) MT1-MMP is a crucial promotor of synovial invasion in human rheumatoid Arthritis Arthritis Rheum. 26;60(3):686-697.

Vaughan-Thomas A, Dudhia J, Bayliss MT, Kadler KE and Duance VC. (2008) Modification of the composition of articular cartilage collagen fibrils with increasing age. Connect Tissue Res. 49(5):374-82.

Richardson LE, Dudhia J, Clegg PD and Smith R (2007) Stem cells in veterinary medicine – attempts at regenerating equine tendon after injury. Trends Biotechnol. 9:409-416.

Dudhia J, Scott CM, Draper ERC, Heinegård D, Pitsillides AA, and Smith RK. (2007) Ageing enhances a mechanically-induced reduction in tendon strength by an active process involving metalloproteinase activity. Ageing Cell 6:547-556.

Cappello R, Bird J, Pfeiffer D, Bayliss MT and Dudhia J (2006) Notochordal cells produce and assemble extracellular matrix in a distinct manner, which may be responsible for the maintenance of healthy nucleus pulposus. Spine 31(8):873-882 (2.676).

Dudhia J (2005) Aggrecan, aging and assembly in articular cartilage. Cell Mol Life Sci. 62(19-20):2241-56.

 

Jay teaches on the BSc in BioVeterinary Sciences, BVetMed and Gateway to BVetMed programmes on extracellular matrix biology, development, structure and function of cartilage and tendons and associated pathologies in man and equids. He also offers and supervises laboratory based projects for BSc and BVetMed undergraduates. He holds a Postgraduate Certificate in Veterinary Education from the RVC and is a Member of the Higher Education Academy. He lectures and examines at UCL, Institute of Orthopaedics for Intercalated BSc, MSc and distance learning programmes.

 

Jay is particulalry interested in clinical appliations of his research and has successful collaborations with both veterinary clinicians within the RVC equine hospital and medical colleagues outside. More recently he has been developing a programme of research with small animal clinicians on applications of stem cells in feline and canine myocardial diseases.

 

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