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DiffQuik Staining Procedure

Solutions required

  1. Fixative (Fast green in methanol) - pale green colour
  2. Stain solution 1 (Eosin G in phosphate buffer) - red colour
  3. Stain solution 2 (Thiazine dye in phosphate buffer) - blue colour

Method

  1. Allow smears to dry
  2. Dip slide or tape-strip five times, for one second each, into Fixative. Allow excess to drain after each dip.
  3. Dip slide or tape-strip five times, for one second each, into Stain 1. Allow excess to drain after each dip.
  4. Dip slide or tape-strip five times, for one second each, into Stain 2. Allow excess to drain after each dip.
  5. Rinse slide or tape-strip in distilled water or Weise's buffer, pH 7.2.
  6. Stick tape strip to slide (sticky side down) and remove excess tape.
  7. Blot or allow to dry in air.
  8. Examine at low power to identify structures and then under oil immersion.

    Staining a tape-strip (426Kb)

Results

  • Yeasts, bacteria and squames generally stain blue
  • Inflammatory cells stain as for Romanowsky stains

Note

  • Microflora and fauna
  • Morphology and organisation of squames
  • Inflammatory cells
  • Hairs, fibres etc.


 
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