In addition to basic research studies, the laboratory provides a comprehensive diagnostic histopathology service for the evaluation of equine neuromuscular disease. Muscle biopsy samples are examined using a variety of histochemical and immunohistochemical techniques. Small animal (dog and cat) muscle samples are also received and evaluated in collaboration with Dr. Diane Shelton's laboratory in California. Atypical myopathy testing is now available for the toxin known to cause this disorder. Both serum and plant samples can be tested. Lactate and pyruvate testing is available through a collaboration with Great Ormond Street NHS foundation trust. Carnitine and organic acid profile assays are evaluated in collaboration with Sheffield Children's NHS foundation trust. For mitochondrial enzyme analysis and mutational screening, please contact the laboratory.Vets with clinical queries should contact Richard Piercy at the Equine Referral Hospital. Trainers and horse owners should contact the laboratory indirectly via their own veterinary surgeon.Equine muscle biopsy results generally are available in 10 working days and small animal results in 3 weeks.
A podcast that covers recent developments in the field of equine exertional rhabdomyolysis is available free via iTunes: please follow this link and scroll down to RVC 32.
For shipment of samples from outside the UK please include an import licence both inside and outside the packaging.
Links to our privacy notices for both clients and veterinary practices can be found here
All of our submission forms, prices and address label are now available within each test section below.
Muscle Biopsy Histopathology
For a thorough pathological evaluation we require a fresh muscle biopsy that is sent to the laboratory chilled (on ice packs). Keeping the sample cold, but not frozen is crucial to avoid artefact. Although we also suggest that you send a biopsy sample fixed in formalin, this latter sample is only used as a back up, in case of unexpected delays. Many routine tests cannot be performed on formalin-fixed muscle. Use a reliable courier for same day/ overnight delivery.
Please contact the laboratory at least a day in advance to ensure we know when to expect delivery. Samples should not be sent out on a friday as they cannot be processed over the weekend and will be wasted. We recommend that you contact a reliable courier for shipment prior to obtaining the sample.
Please collect and package muscle biopsy samples according to our muscle biopsy procedure and send to the laboratory in a clearly labelled polystyrene box.
We recommend you send in a sample of blood in EDTA (approximately 10ml) and in a plain tube as we bank DNA samples from horses with neuromuscular diseases and in case of further testing. Client and horse details will remain confidential. Blood samples can be sent together with muscle samples in the same package
Equine Muscle Biopsy Evaluation
We have two different levels of muscle sample evaluation; a basic and full panel.
Our basic panel includes routine histological staining which looks at muscle fibre morphology and glycogen content. It is often suitable for initial differential diagnoses of recurrent exertional myopathies and polysaccharide storage myopathy however, in some circumstances we may subsequently advise further testing.
The first muscle is charged at £100 (exc. VAT) and additional samples from the same horse submitted at the same time are charged at £44.50 (exc. VAT)
Our full panel offers the most detailed evaluation of biopsy samples encompassing all of the basic panel plus special histopathological staining, immunohistochemistry and oxidative stains to assess lipid content, fibre types & mitochondrial enzymes. It is suitable for all cases and those requiring a more in depth investigation, including differential diagnoses of other myopathies and neuropathies including equine motor neurone disease, atypical myopathy and suspected mitochondrial defects.
The first muscle is charged at £252 (exc. VAT) and additional samples from the same horse submitted at the same time are charged at £98 (exc. VAT)
Small animal Biopsy Evaluation
In collaboration with Diane Shelton's laboratory at UCSD small animal muscle and nerve samples are evaluated. Serum antibody tests for myasthenia gravis (AChR) and masticatory muscle myositis (2M) can also be run- Collect 1-2mls of serum prior to initiation of corticosteroid or other immunosuppressive therapy. A full list of tests available and their prices (excluding VAT) are available below:
Muscle or Nerve Profile: £252
Combined Muscle and Nerve Profile: £283.50
Additional Muscle or Nerve Profile (sent in at the same time form the same animal): £102
2M Antibody test: £95
AChR Antibody test: £95
The required downloadable and printable forms for submitting muscle biopsies from both horses and small animals are available below:
PSSM1 (Polysaccharide storage myopathy) Genetic Testing
Atypical Myopathy Testing for Vets and Owners
Following research that was supported by The Horse Trust, we now offer vets and owners atypical myopathy testing.
Serum testing for the hypoglycin A toxin and its principal metabolite known to cause atypical myopathy is now available. This is a more rapid test than previously reported methods. We also offer combined profiles including hypoglycin A & acylcarnitine identification and hypoglycin A, acylcarnitine & organic acid identification at special prices. Please send either a whole blood tube, serum gel tube or separated serum using a next day delivery service and use the specific form below. For organic acids profiles please send a urine sample and for acylcarnitines please send a lithium heparin blood tube.
Whenever possible, samples received before 9am will be reported in 48 hours and samples received after 9am will be reported in 72 hours. Please note these turnaround times are for working days only (the lab is open Monday-Friday)
Prices (all excluding VAT) of the available atypical myopathy tests are as follows:
Serum hypoglycin A & MCPA- carnitine: £95
Serum hypoglycin A & MCPA- carnitine (6 or more samples): £85 per sample
Serum hypoglycin A, MCPA- carnitine & acylcarnitine profile: £179
Full atypical myopathy profile (HGA, MCPA-c, organic acids & acylcarnitine profiles): £275.
Samples from overseas: Samples from abroad can be sent to us. The vet will need to send whole blood (e.g red topped vacutainer tube) or if they have the facility to spin down the blood, just the serum can be sent. The vet will need to send samples on a next day delivery (as sample decomposition can affect accuracy of results) only Monday-Thursday. It must include one of our import licences with the courier paperwork. The sample should be packaged correctly i.e if using a padded envelope the blood tube must be surrounded by enough absorbent material to soak up all of the tube contents and this should all be in a sealed bag. The paperwork should be separate from the bagged tube.
If the vet takes a sample that cannot be sent immediately e.g. on a Friday, the sample should be spun, the serum placed into a new tube and frozen until ready to send. When sending samples that have been frozen, they do not need to be defrosted before being sent, the package will need to include ice packs and preferably be in an insulated container that will keep the tube cold.
For horse owners
We offer testing of seeds, leaves and seedlings for the Hypoglycin A toxin known to cause atypical myopathy. To find out if plant samples on your property contain the toxin owners can submit these samples directly to the lab. Please read the atypicla myopathy information sheet below to learn more on atypical myopathy and how to collect samples. Payment is required upon submission via debit/credit card or cheque. Please complete the owner's plant sample submission form below which also contains debit/credit card payment details If paying by cheque please make payable to Royal Veterinary College. Results are available in 2-4 weeks following submission however if you require a more rapid turnaround we offer an expedited service (at a higher cost) and results are, whenever possible, available within 48-72 hours. These turnaround times are for working days only and we can only accept credit/debit card payments for the expedited service (please fill out details on the second page of the plant sample submission form).
A Standard turnaround plant / tree sample test costs £60 (inc VAT) per sample
The expedited plant / tree sample test costs £150 (inc VAT) per sample
Horse owner factsheet and sample guidelines Download here
Unfortunately, due to staffing limitations, the Comparative Neuromuscular Diseases Laboratory is unable to discuss individual results or atypical myopathy testing in general by telephone or email. We do provide generic advice on each result sheet about how to interpret your test result and give advice and information about atypical myopathy on our owner information sheet as well as in the frequently asked questions (FAQ) document Please take some time to look through these. We recommend you contact your own vet if you have specific further queries.
Lactate Pyruvate Ratios
Please follow the protocol for precipitation of samples for lactate pyruvate ratios to ensure samples are correctly processed and complete the specific submission form for this type of sample available below. Samples must be sent to the laboratory on dry ice to avoid thawing using either a same day or next day delivery courier. Please contact the laboratory at least a day in advance to ensure we know when to expect delivery. Samples should not be sent out on a friday as they cannot be processed over the weekend and will be wasted. We recommend that you contact a reliable courier for shipment prior to obtaining the sample. Prices for lactate & pyruvate testing are also listed below.
First sample (e.g. Pre-exercise sample): £107
Second sample (e.g. Post-exercise sample): £36
Acylcarnitine and Organic acids profiles
Mitochondrial respiratory chain enzyme analysis and Mutational screening
Please contact the laboratory to discuss your requirements.