Department: Pathobiology and Population Sciences

Campus: Hawkshead

Research Groups: Safe and Sustainable Food

Research Centres: RVC Quantitative Biology Resource

Muhammad is a Senior Lecturer in Animal Reproduction and a member of the Reproduction Research Group.

Muhammad is trained as a veterinarian from the College of Veterinary Sciences, Lahore and had MSc (Hons) in Animal Reproduction before he obtained his PhD from the University of Nottingham.  For his PhD he worked on the control of gonadotrophins release under the supervision of Professor William Haresign. His interest in neuroendocrinology made him to move to the University of Illinois, Urbana-Champaign, USA to work with Professor Gary Jackson on Control of GnRH release using Push-pull perfusion technique. This was followed by teaching as Assistant Professor in the college of Veterinary Sciences, Lahore for 3 years.  Subsequently he joined university of Nottingham as Research fellow to work on defective luteal function in ruminants before moving to the Royal Veterinary College in 1999.  At the RVC, his research has expanded to include urinary incontinence in the dog along with different aspects of ruminant reproduction.  Muhammad is a member of the editorial boards of "The International Journal of Veterinary Surgery and Medicine and "Pakistan Veterinary Journal." Muhammad is a Diplomat of European College of Animal Reproduction (ECAR).

Muhammad's main research interests are in the general area of animal reproduction:

  • Role of angiogenic growth factors in the corpus luteum development investigating how the pre-ovulatory LH surge and/or progesterone exposure contribute to the angiogenic growth factors’ expression in the post-ovulatory follicle and/or corpus luteum development. The long-term aim is to develop methodologies to control the timing of the post-ovulatory rise in progesterone to reduce early embryonic death.

  • Molecular and cellular aspects of the ovine cervical function, in general and the role of the extracellular matrix (ECM) in remodelling the cervix during relaxation, in particular.The long-term aim is to develop novel approaches to facilitate trans-cervical intrauterine insemination without compromising fertility not only in sheep but also other species including humans and endangered animal species.

  • Mechanism of development of urinary incontinence in the dog investigating the role of gonadotrophins and/or their receptors in the development of urethral sphincter mechanism incompetence (USMI).

Publications since 2010:

Akhter S, Rakha BA, Ansari MS, Iqbal S, Khalid M (2018)  Evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (Bubalus bubalis) bull spermatozoa. Theriogenology, 2018 Jun 23;119:137-142. doi: 10.1016/j.theriogenology.2018.06.019

Husna AU, Azam A, Qadeer S, Awan MA, Nasreen S, Shahzad Q, Fouladi-Nashta AA, Khalid M, Akhter S., (2017) Sperm preparation through Sephadex™ filtration improves in vitro fertilization rate of buffalo oocytes. Reprod Domest Anim. 2017 Dec 14. doi: 10.1111/rda.13117.

Azam A, Shahzad Q, Husna AU, Qadeer S, Ijaz R, Fouladi-Nashta AA, Khalid M, Ullah N, Akhtar T, Akhter S (2017) Supplementing α-Linolenic acid in the in vitro maturation media improves nuclear maturation rate of oocytes and early embryonic development in the Nili Ravi buffalo.  Anim. Reprod., 14: 1161-1169

Akhter S, Rakha BA, Ansari MS, Husna AU, Iqbal S and Khalid M (2017) Evaluation of Quail and Turkey egg yolk for cryopreservation of Nili-Ravi Buffalo bull semen. Theriogenology, 87: 259-265

Adams J and Khalid M (2017) Ewe reproduction: stimulation and synchronisation.  Livestock, 21: 236-239

Mehl NS, Srisuwatanasagul S, Swangchan-Uthai T, Sirivaidyapong S, Khalid M. (2017)  GnRH-agonist implants suppress reproductive function and affects ovarian LHR and FSHR expression in prepubertal female cats.  Theriogenology, 87:250-258.

Leethongdee S, Khalid M, Scaramuzzi RJ.(2016) The effect of the intracervical administration of FSH or LH on the levels of hyaluronan, COX2, and COX2 mRNA in the cervix of the nonpregnant ewe.  Theriogenology. 2016 Dec;86(9):2244-2253. doi: 10.1016/j.theriogenology.2016.07.014

Waleed F.A. Marei, Kabir A. Raheem, Mazdak Salavati, Tina Tremaine, Muhammad Khalid, Ali A. Fouladi-Nashta (2016)  Hyaluronan and hyaluronidase, which is better for embryo development?  Theriogenology, 86:940-8.

Akhter S, Rakha BA, Ansari MS, Husna AU, Iqbal S, Khalid M. (2016)  Evaluation of quail and turkey egg yolk for cryopreservation of Nili-Ravi buffalo bull semen.  Theriogenology. 2016 Sep 9. pii: S0093-691X(16)30414-9. doi: 10.1016/j.theriogenology.2016.09.002.

Mehl NS, Khalid M, Srisuwatanasagul S, Swangchan-uthai T, Sirivaidyapong S. (2016)  GnRH-agonist implantation of prepubertal male cats affects their reproductive performance and testicular LH receptor and FSH receptor expression.  Theriogenology. 2016 Mar 15;85(5):841-8. doi: 10.1016/j.theriogenology.2015.10.031

Khalil, WA, Marei WF, Farooq H, Khalid M (2014)  A comparison between the effect of linoleic acid and conjugated linoleic acid on bovine oocyte developmental competence.  J. Animal and Poultry Prod., Mansoura Univ., Vol.5 (3): 115 -126, 2014

Leethongdee S, Khalid M, Scaramuzzi R (2014) The Effect of the Intracervical Application of Follicle-Stimulating Hormone or Luteinizing Hormone on the Pattern of Expression of Gonadotrophin Receptors in the Cervix of Non-Pregnant Ewes.  Reprod Domest Anim. 2014 Apr 23. doi: 10.1111/rda.12312. [Epub ahead of print]

Christensen AC, Haresign W, Khalid M (2014)  Progesterone exposure of seasonally anoestrous ewes alters the expression of angiogenic growth factors in preovulatory follicles.  Theriogenology. 2014 Jan 15;81(2):358-67.

Linharattanaruksa P, Srisuwatanasagul S, Ponglowhapan S, Khalid M, Chatdarong K (2014)  Collagen and glycosaminoglycan profiles in the canine cervix during different stages of the estrous cycle and in open- and closed-cervix pyometra.  J Vet Med Sci. 2014 Mar 1;76(2):197-203.

Khalil WA, Marei WF, Khalid M (2013) Protective effects of antioxidants on linoleic acid-treated bovine oocytes during maturation and subsequent embryo development.  Theriogenology. 80: 161-168.

Raheem KA, Marei WF, Mifsud K, Khalid M, Wathes DC, Fouladi-Nashta AA (2013)  Regulation of the hyaluronan system in ovine endometrium by ovarian steroids.  Reproduction, 145:491-504.

Somchit-Assavacheep A, Campbell BK, Khalid M, Kendall NR, Scaramuzzi RJ (2013)  The effect of short-term nutritional supplementation of ewes with lupin grain (Lupinus luteus) on folliculogenesis, the concentrations of hormones and glucose in plasma and follicular fluid and the follicular levels of P450 aromatase and IRS-1, -2 and -4.  Reproduction, 145: 319-33.

Christensen AC, Haresign W, Khalid M (2012) Progesterone exposure of the preovulatory follicle in the seasonally anoestrous ewe alters the expression of angiogenic growth factors in the early corpus luteum.  Theriogenology. 77:1648-1660.

Perry K, Haresign W, Wathes DC, Pitsillides AA, Khalid M (2012) Cervical expression of hyaluronan synthases varies with the stage of the oestrous cycle in the ewe.  Theriogenology, 77:1100-1110.

Ponglowhapan S, Church DB, Khalid M (2012)  Expression of luteinizing and follicle-stimulating hormone receptor in the dog prostate.  Theriogenology, 78:777-783.

Akhter S, Ansari MS, Rakha BA, Andrabi SMK, Rakha BA, Ullah N and Khalid M (2012)  Soya-lecithin in extender improves the freezability and fertility of buffalo (Bubalus bubalis) bull spermatozoa.  Domestic Animal Reproduction 47:815-819

Ponglowhapan S, Church DB, Khalid M (2011)  Effect of the gonadal status and the gender on glycosaminoglycans profile in the lower urinary tract of dogs.  Theriogenology, 76(7):1284-92.

Akhter S, Ansari MS, Rakha BA, Andrabi SMK, Khalid M, Ullah N (2011)  Effect of low density lipoproteins in extender on freezability and fertility of buffalo (Bubalus bubalis) bull semen. Theriogenology  76:759-64

Perry K, Haresign W, Wathes DC and Khalid M (2010)  Intracervical application of hyaluronan improves cervical relaxation in the ewe.  Theriogenology  74:1685-90

Ansari MS , Rakha BA, Ullah N, Andrabi SMH, Iqbal S, Khalid M and Akhter S (2010)  Effect of exogenous glutathione in extender on the freezability of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa.  Animal Science Papers and Reports, 28: 235-244.

Ponglowhapan S, Church DB, Khalid M (2010) Expression of prostaglandin E2 receptor subtypes in the canine lower urinary tract varies according to the gonadal status and gender.  Theriogenology. 2010 doi:10.1016/j.theriogenology.2010.06.017

Leethongdee S, Kershaw-Young CM, Scaramuzzi RJ and Khalid M  (2010)  Intra-cervical application of Misoprostol at estrus alters the content of cervical hyaluronan and the mRNA expression of follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR) and cyclooxygenase-2 in the ewe.  Theriogenology, 73:1257-1266.

Perry K, Haresign W, Wathes DC and Khalid M (2010)  Hyaluronan (HA) content, the ratio of HA fragments, and the expression of CD44 in the ovine cervix varies with the stage of the oestrous cycle.  Reproduction 140:133-141.

Kershaw-Young CM, Scaramuzzi RJ, McGowan MR, Pitsillides AA, Wheeler-Jones CPD and M Khalid (2010)  The effect of oestradiol on COX-2, EP2 and EP4 mRNA expression and the extracellular matrix in the cervix of the hypogonadotrophic ovariectomised ewe.  Theriogenology, 73: 620-628.

Akhter S, Ansari MS , Rakha BA, Ullah N, Andrabi SMH and Khalid M (2010)  In vitro evaluation of liquid storage buffalo semen at 5°C diluted in soya lecithin based extender (Bioxcell®), tris-citric egg yolk, skim milk and egg yolk-citrate extenders.  Reproduction in Domestic Animals, 46: 45-49.  DOI: 10.1111/j.1439-0531.2009.01561.x


Presentations at International Conferences:

Kafi A, Herath T, Khalid M and Kershaw C (2018)  Cysteine supplementation pre- freeze and post- thaw improves integrity and reduces oxidative stress in cryopreserved ram spermatozoa.  Abstract # P144, Proceedings of the 22nd Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR) Cordoba, Spain, 27 – 29 September 2018

Kafi A, Herath T, Khalid M and Kershaw C (2018)  Improving the integrity of frozen-thawed ram semen with seminal plasma proteins.  Poster presentation in Fertility 2018 conference in Liverpool (3-6 January 2018).

Khalid M (2016) Transcervical AI in Sheep: Problem to Solution, Presented at the Chulalongkorn University Veterinary Congress, Bangkok, Thailand 20-22 April 2016.

Khalid M (2016) Defective Corpus Luteum: the underlying mechanism,  Presented at the Chulalongkorn University Veterinary Congress, Bangkok, Thailand 20-22 April 2016.

Nicole Sirisopit Mehl, Muhammad Khalid, Sayamon Srisuwatanasagul, Theerawat Swangchan-Uthai, and Sudson Sirivaidyapong  (2016) Comparison of the ovarian and uterine reproductive parameters, and the ovarian mRNA and protein expression of LHR and FSHR between the prepubertal and adult female cats. Presented at the 8th Quadrennial International Symposium of Canine and Feline Reproduction (ISCFR 2016 - EVSSAR 2016) JUNE 22 – 25, 2016, Paris, France.

Y. Akar, N. Ahmad and M. Khalid (2016) The Effect of cadmium on the bovine oocyte maturation and developmental competence of embryo.  Presented at the 18th International Conference on Animal Reproduction (ICAR) June 26-30th 2016, Tours, France

KL Perry, Haresign W, Khalid M (2016) The signalling mechanism of Hyaluronan to induce cervical relaxation in the ewe.  Presented at the 18th International Conference on Animal Reproduction (ICAR) June 26-30th 2016, Tours, France.

N.S.Mehl, M. Khalid, S. Srisuwatanasagul, and S. Sirivaidyapong (2015) GnRH-agonist implantation in pre-pubertal female cats does not alter their gonadal LHR protein expression. 14th Chulalongkorn University Veterinary Conference. 20-22 April 2015. Thailand.

N.S.Mehl, M. Khalid, T. Swangchan-Uthai, and S. Sirivaidyapong (2015) GnRH-agonist implantation of pre-pubertal male cats does not alter their testicular LHR mRNA expression. RGJ-Ph.D. Congress XVI "ASEAN: Emerging Research Opportunities." 11 - 13 June 2015. Pattaya, Chonburi, Thailand.

Mehl N.S., Srisuwatanasagul S., Sirivaidyapong S., Khalid M (2015) GnRH-agonist implantation of pre-pubertal male cats affects their testiculatal LHR expression. The 40th World Small Animal Veterinary Association Congress (WSAVA 2015). 15 - 18 May 2015. Bangkok, Thailand

Tremaine T, Fouladi-Nashta AA, Wathes DC and Khalid M (2015) A potential co-culture system of ovine blastocyst with uterine endometrium to mimic initial attachment of embryo implantation.  Abstract number O007, Presented at the Society for Reproduction and Fertility, 2015SRF Conference and Exhibition, St Catherine’s College, Oxford, 20-22 July 2015, UK.

Muhammad lectures to BVM1 on sheep health and production, to BVM3 on different aspects of clinical reproduction and contributes to clinical rotational teaching to BVM5. Muhammad is Reproduction strand leader.

Muhammad is often asked to speak to veterinary audiences internationally in countries like Thailand, Pakistan, Turkey and Bangladesh on his work on sheep reproduction.

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