Oliver Garden
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Oliver is a Senior Lecturer in Internal Medicine and Molecular Immunology. His research focuses on regulatory T cells and autoimmune disease in dogs, mice and human beings.
Biography
Oliver graduated from the Royal Veterinary College (RVC) in 1993, having completed an intercalated BSc at King's College London in 1990. Following a rotating internship in small animal medicine and surgery, he successfully completed a Wellcome Trust Clinical PhD programme in small animal gastroenterology and immunology at the RVC in 1998 and then a Wellcome Trust Research Training Fellowship at the University of South Carolina Medical School in 1999.
From 1999 to 2001, Oliver completed a Residency in Small Animal Internal Medicine at Cornell University. Between 2001 and 2004, Oliver undertook a Wellcome Trust Advanced Fellowship within the Department of Immunology at Imperial College London (ICL), Hammersmith Campus. He also gained the DipACVIM in 2002 and the DipECVIM-CA in 2003. Oliver became an Honorary Lecturer in the Department of Immunology at ICL in 2003, and from 2004 has held a dual appointment with the RVC as a Senior Lecturer in Internal Medicine and Molecular Immunology, and with ICL as a Research Lecturer.
Research
Dr Oliver Garden’s laboratory, based at the Camden Campus of the Royal Veterinary College, specialises in cellular immunology, with a specific expertise in regulatory T cell (Treg) biology. Oliver started his laboratory with the aid of a Wellcome Trust Advanced Fellowship in 2001 within the Department of Immunology, Imperial College London, Hammersmith Campus, following post-doctoral training at the University of South Carolina Medical School and a Residency in Small Animal Internal Medicine at Cornell University.
To date, two PhD students, two BSc students and six MSc students have successfully graduated following projects carried out within Oliver’s laboratory; of the MSc students, two have completed PhD degrees and three are currently undertaking PhD studies. The laboratory has also employed two Research Technicians, who have both moved on to prestigious Research Associate posts – one at King’s College London and the other at University College London – following the conclusion of their contracts. The laboratory is currently home to two PhD students and hosts from one to three undergraduate or postgraduate students – undertaking BSc, BVetMed or MSc degrees – at any one time.
Key techniques for which Oliver’s laboratory has specific expertise include T cell culture, including sophisticated Treg assays in vitro; multi-colour analytical flow cytometry; ELISAs of a number of Th1 and Th2 cytokines; and molecular biological techniques, including PCR, cloning, transduction of constructs into mammalian cells and spectratyping. Funding for work in Oliver’s laboratory has been provided by the Wellcome Trust and Medical Research Council, and currently includes grants from the Biotechnology and Biological Sciences Research Council, European College of Veterinary Internal Medicine, and American College of Veterinary Emergency and Critical Care.
Oliver’s laboratory has successfully worked with three major industrial partners over the past six years, consistently delivering on-time and within budget – for both product-orientated projects and blue-sky research. Current industrial sponsors include Novartis and Alba Therapeutics. The laboratory has a dynamic, forward-looking, innovative and committed work ethic – with an unquestionable dedication to its team members, to its academic funders and to its industrial partners.
Teaching
Oliver is a Fellow of the Higher Education Academy, having completed the Certificate of Advanced Studies in Learning and Teaching at Imperial College London (ICL) in 2005. He teaches at both the RVC and ICL. His teaching portfolio at the RVC includes lectures on feline infectious diseases, delivered to the Fourth Year BVetMed students; a role as leader of the Feline Medicine Elective Module, on which he lectures and presents cases in the format of problem-based learning; and both structured and ad hoc teaching on the clinic floor of the Queen Mother Hospital for Animals.
His teaching responsibilities at ICL include lectures on dendritic cells, immunological tolerance, Th1/2/17 cells, regulatory T cells and systemic lupus erythematosus. He presents to both the medical students undertaking the integrated BSc degree and to the MSc students within the Department of Immunology, acting as both a Course Tutor and examiner for the MSc degree. His particular pedagogical interest is maximising the effectiveness of ad hoc clinical teaching on the hospital floor..
Selected Publications
GAJANAYAKE, I, PRIESTNALL, SL, BENIGNI, L, ENGLISH, K, SUMMERS, B AND GARDEN, OA (2010) Paraneoplastic hypercalcemia in a dog with benign renal angiomyxoma. Journal of Veterinary Diagnostic Investigation (in the press)
ALLENSPACH, K, HOUSE, A, SMITH, K, MNNEILL, FM, HENDRICKS, A, ELSON-RIGGINS, J, RIDDLE, A, STEINER, JM, WERLING, D, GARDEN, OA, CATCHPOLE, B AND SUCHODOLSKI, JS (2010) Evaluation of mucosal bacteria and histopathology, clinical disease activity and expression of Toll-like receptors in German shepherd dogs with chronic enteropathies. Veterinary Microbiology (in the press)
CARLUCCI, F, FOSSATI-JIMACK, L, DUMITRIU, IE, HEIDARI, Y, WALPORT, MJ, SZAJNA, M, BARUAH, P, GARDEN, OA, COOK, HT AND BOTTO, M (2010) Identification and characterization of a lupus suppressor 129 locus on chromosome 3. Journal of Immunology 184(11): 6256-6265
FERREIRA, C, SINGH, Y, FURMANSKI, AL, WONG, FS, GARDEN, OA AND DYSON, J (2009) Non-obese diabetic mice select a low-diversity repertoire of natural regulatory T cells. Proceedings of the National Academy of Sciences (USA) 106(20): 8320-8325
KATHRANI, A, STEINER, JM, SUCHODOLSKI, J, EASTWOOD, J, SYME, H, GARDEN, OA, ALLENSPACH, K (2009) Elevated canine pancreatic lipase immunoreactivity concentration in dogs with inflammatory bowel disease is associated with a negative outcome. Journal of Small Animal Practice 50(3): 126-132
KOHLHAAS, S, GARDEN, OA, SCUDAMORE, C, TURNER, M, OKKENHAUG, K AND VIGORITO, E (2009) Cutting Edge: the Foxp3 target miR-155 contributes to the development of regulatory T cells. Journal of Immunology 182(5): 2578-2582 (dual first authorship)
HASLAM, S.M., JULIEN, S, BURCHELL, J.M., MONK, C.R., CERONI, A., GARDEN, O. A. and DELL, A (2008) Characterizing the glycome of the mammalian immune system. Immunology and Cell Biology
ISAACS, J. D., GARDEN, O. A., KAUR, G., COLLINGE, J., JACKSON, G. S. & ALTMANN, D. M. (2008) The cellular prion protein is preferentially expressed by CD4(+) CD25(+) Foxp3(+) regulatory T cells. Immunology. PubMed ID 18462346
YATES, J., ROVIS, F., MITCHELL, P., AFZALI, B., TSANG, J. Y., GARIN, M., LECHLER, R. I., LOMBARDI, G. & GARDEN, O. A. (2007) The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol 19, 785-799. PubMed ID 17545278
GARDEN, OA, REYNOLDS, PR, YATES, J, LARKMAN DJ, MARELLI-BERG FM, HASKARD DO, EDWARDS AD AND GEORGE AJT (2006) A rapid method for labelling CD4+ T cells with ultrasmall paramagnetic iron oxide nanoparticles for magnetic resonance imaging that preserves proliferative, regulatory and migratory behaviour in vitro. Journal of Immunological Methods 314(1-2): 123-133
PATTON, D. T., GARDEN, O. A., PEARCE, W. P., CLOUGH, L. E., MONK, C. R., LEUNG, E., ROWAN, W. C., SANCHO, S., WALKER, L. S., VANHAESEBROECK, B. & OKKENHAUG, K. (2006) Cutting edge: the phosphoinositide 3-kinase p110 delta is critical for the function of CD4+CD25+Foxp3+ regulatory T cells. J Immunol 177, 6598-6602. PubMed ID 17082571
MONK, C. R., SUTTON-SMITH, M., DELL, A. & GARDEN, O. A. (2006) Preparation of CD25(+) and CD25(-) CD4(+) T cells for glycomic analysis--a cautionary tale of serum glycoprotein sequestration. Glycobiology 16, 11G-13G. PubMed ID 16770844
GARDEN, O. A., REYNOLDS, P. R., YATES, J., LARKMAN, D. J., MARELLI-BERG, F. M., HASKARD, D. O., EDWARDS, A. D. & GEORGE, A. J. (2006) A rapid method for labelling CD4+ T cells with ultrasmall paramagnetic iron oxide nanoparticles for magnetic resonance imaging that preserves proliferative, regulatory and migratory behaviour in vitro. J Immunol Methods 314, 123-133. PubMed ID 16860821
MONK, C. R., SPACHIDOU, M., ROVIS, F., LEUNG, E., BOTTO, M., LECHLER, R. I. & GARDEN, O. A. (2005) MRL/Mp CD4+,CD25- T cells show reduced sensitivity to suppression by CD4+,CD25+ regulatory T cells in vitro: a novel defect of T cell regulation in systemic lupus erythematosus. Arthritis Rheum 52, 1180-1184. PubMed ID 15818683
ROVIS, F, WAIT, R, LEE, BBC, MONK, CR, LECHLER, RI AND GARDEN, OA (2004) Proteomic characterization of murine CD4+CD25+ regulatory T cells. Immunology 2004. Vol. ISBN 88-7587-069-1, pp. 79-84. Bologna: Medimond Srl.
LECHLER, R. I., GARDEN, O. A. & TURKA, L. A. (2003) The complementary roles of deletion and regulation in transplantation tolerance. Nat Rev Immunol 3, 147-158. PubMed ID 12563298
GARDEN, OA (2001) Genetic modelling of intestinal disease: the paradigm of gluten-sensitive enteropathy in the Irish setter dog – a model for human coeliac disease? The European Journal of Comparative Gastroenterology 10: 2-12.
RICH, I. N., WORTHINGTON-WHITE, D., GARDEN, O. A. & MUSK, P. (2000) Apoptosis of leukemic cells accompanies reduction in intracellular pH after targeted inhibition of the Na(+)/H(+) exchanger. Blood 95, 1427-1434. PubMed ID 10666221
GARDEN, O. A., PIDDUCK, H., LAKHANI, K. H., WALKER, D., WOOD, J. L. & BATT, R. M. (2000) Inheritance of gluten-sensitive enteropathy in Irish Setters. Am J Vet Res 61, 462-468. PubMed ID 10772115
GARDEN, O. A., MUSK, P., WORTHINGTON-WHITE, D. A., DEWEY, M. J. & RICH, I. N. (2000) Silent polymorphisms within the coding region of human sodium/hydrogen exchanger isoform-1 cDNA in peripheral blood mononuclear cells of leukemia patients: A comparison with healthy controls. Cancer Genet Cytogenet 120, 37-43. PubMed ID 10913675