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DiffQuik Staining Procedure
Solutions required
- Fixative (Fast green in methanol) - pale
green colour
- Stain solution 1 (Eosin G in phosphate buffer) - red
colour
- Stain solution 2 (Thiazine dye in phosphate buffer) - blue
colour
Method
- Allow smears to dry
- Dip slide or tape-strip five times, for one second each, into
Fixative. Allow excess to drain after each dip.
- Dip slide or tape-strip five times, for one second each, into
Stain 1. Allow excess to drain after each dip.
- Dip slide or tape-strip five times, for one second each, into
Stain 2. Allow excess to drain after each dip.
- Rinse slide or tape-strip in distilled water or Weise's buffer,
pH 7.2.
- Stick tape strip to slide (sticky side down) and remove excess
tape.
- Blot or allow to dry in air.
- Examine at low power to identify structures and then under oil
immersion.
Staining
a tape-strip (426Kb)
Results
- Yeasts, bacteria and squames generally stain blue
- Inflammatory cells stain as for Romanowsky stains
Note
- Microflora and fauna
- Morphology and organisation of squames
- Inflammatory cells
- Hairs, fibres etc.
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