Recombinant DNA
Prof Peter Chantler
Course: Molecular Biology of the Cell
Year 1
Molecular Cloning
Introduction
Molecular cloning—not to be confused with cell cloning or whole animal cloning—is an important tool in our endeavour to understand the structure, function and regulation of individual genes and their products. An important resource is the creation of a DNA library, which is a complete collection of DNA fragments, each located singly within a cloning vector that is capable of replication when transferred to an appropriate host cell. Two distinct types of library can be formed, depending on whether the cloned DNA was derived by fragmentation of the genome (genomic library) or was copied from cellular messenger RNA (cDNA library). The following basic steps are involved in the molecular cloning of genes.
a. Choice of DNA: genomic DNA or cDNA
b. Insertion of the chosen DNA into a vector
c. Creation of a DNA library
d. Library screening
e. Creation of an expression library
Course
1 Molecular Cloning
a Choice
b Insertion
c DNA Library
d Screening
e Exp Library
2 DNA Sequencing
3 PCR
4 VNTRs
5 RAPD
Contacts
Peter Chantler
Online Learning
Links
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