The Imaging Suite is a purpose-built facility. It contains microscopes capable of experiments ranging from basic microscopy of cells or tissues to complex imaging, including: 3-dimensional reconstructions, live cell imaging, imaging of multiple fluorescent dyes or a combination them.

The facility is run and managed by Dr Andrew Hibbert  who provides full training to all microscope users.

Our microscopes are available for hire to external academic or non-academic users with full training provided or microscopy carried out by Dr Hibbert. (Contact details are at the bottom of the page.)

Equipment and Capabilities

Currently, the following equipment and capabilities are to be found within our Imaging Suite:

Wide field light and fluorescence microscopes

These are used for most normal imaging of cells or tissues, which might be stained with coloured dyes, fluorescent dyes or not stained at all. They can be imaged at scales ranging from entire tissues to details of a few cells.

Our six microscopes are fitted with high-resolution colour or sensitive monochrome cameras. We can image using all of the main microscopy methods through either upright or inverted microscopes, providing great flexibility in the types of sample we can image.

Confocal microscopes

These eliminate out of focus light to image fluorescent dyes in a thin "optical slice", as though a very thin section of your sample had been cut out. By stacking these slices on top of each other three-dimensional reconstructions of your cells or tissues can be created. We have two confocal microscopes in the facility and are able to image all commonly used dyes.

Live imaging

We have a microscope at 37°C with a supply of humidified 5% CO2. This allows imaging of living cells over time courses up to overnight. This microscope can be used for confocal or wide field phase contrast imaging. We also have a system equipped with a sensitive EM-CCD camera and monochromator for rapid imaging of live cells, particularly suited to imaging intracellular calcium. Image analysis

Image analysis

 We have a range of software packages for the manipulation and quantitation of the images you collect in the Suite. Dr Hibbert is experienced in the use of all these programs and available to provide any assistance necessary with presenting or quantifying images. Other RVC “Imaging” Facilities

Other RVC “Imaging” Facilities

The RVC has a wide range of other facilities which can be described as “Imaging”, including:

The diagnostic imaging service 

Histology and cytology services 

The micro CT

FACS machines and flow cytometers 

Further Information and Contact

If you require any further details of any of the equipment capabilities or wish to request booking information then please contact Dr Andrew Hibbert, Imaging Suite Technician, CBS Department, RVC, Royal College Street, London NW1 0TU. Tel: 020 7468 5257 or e-mail ahibbert@rvc.ac.uk

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