Shan Goh
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A Research Fellow in Liam Good's group, Shan has varied interests in antisense RNA for gene silencing in prokaryotes, phage as therapeutic agents and finding novel animal viruses with potential for zoonoses
Biography
Shan completed her BSc(Hons.) majoring in Microbiology and Pathology at the University of Western Australia, and continued postgraduate studies in the same university on bacteriophages of Clostridium difficile. She obtained a PhD in 2004. She then did postdoctoral studies in Singapore at the National University of Singapore where she continued work on C. difficile phages, as well as at Bioprocessing Technology Institute, A*STAR, where she constructed expression plasmids for an actinomycete. In 2006, she was awarded a visiting scientist scholarship by the Swedish Institute for postdoctoral studies in E. coli essential genes by RNA silencing at Karolinska Institute in Stockholm, Sweden. In addition, she explored the use of 454 sequencing for detection of novel viruses in humans. Since May of 2009, Shan has joined the RVC and is continuing her search for novel viruses in animals, expanding her antisense RNA skills to more bacteria and reviving her interest in phages.
Research
RNA silencing in prokaryotes, particularly for the study of growth essential genes; virus discovery in veterinary systems and in the context of One Health; bacteriophage therapy as an alternative to antibiotic therapy; non-antibiotic cloning and protein expression systems.Selected Publications
Goh S, Boberek JM, Nakashima N, Stach J & Good L. (2009). Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli. PLoS One 4(6):e6061.
Goh S, Lindau C, Tiveljung-Lindell A & Allander T. (2009). Merkel cell polyomavirus in respiratory tract secretions. Emerging Infectious Diseases 15(3):489-91.
Lindau C, Tiveljung-Lindell A, Goh S, Ramqvist T & Allander T. (2009). A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses. J Clin Virol 44(1):24-6.
Goh S & Good L. (2008). Plasmid selection in Escherichia coli using an endogenous essential gene marker. BMC Biotechnology Aug11;8(1):61.
Goh S, Camattari A, Ng D, Song R, Madden K, Westpheling J & Wong VV. (2007). An integrative expression vector for Actinosynnema pretiosum. BMC Biotechnology; Oct 24; 7:72.
Nikravesh A, Dryselius R, Faridani OR, Goh S, Sadeghizadeh M, Behmanesh M, Ganyu A, Klok EJ, Zain R, & Good L. (2007). Antisense PNA accumulates in Escherichia coli and mediates a long post antibiotic effect. Molecular Therapy; 15(8):1537-42.
Goh S, Ong PF, Song KP, Riley TV & Chang BJ (2007). The complete genome sequence of Clostridium difficile phage phiC2 and comparisons to phiCD119 and inducible prophages of CD630. Microbiology; 153: 676-685.
Dryselius R, Nikravesh A, Kulyte A, Goh S & Good L. (2006). Variable coordination of cotranscribed genes in Escherichia coli following antisense repression. BMC Microbiol; 21; 6:97.
Goh S, Riley TV & Chang BJ. (2005). Isolation and characterization of temperate bacteriophages of Clostridium difficile. Appl Environ Microbiol; 71:1079-1083.
Goh S, Chang BJ & Riley TV. (2005). Effect of phage infection on toxin production by Clostridium difficile. J Med Microbiol; 54(Pt 2):129-35.